Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit MANF/ARMET Polyclonal Antibody | anti-MANF/ARMET antibody
Anti-MANF/ARMET Antibody
Predicted: Mouse, Rat
Predicted: Mouse, Rat
IHC: 1:50-1:100
IHC-P: 1:100
WB: 1:500-1:2000
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using MANF antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using MANF antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
Uniprot Description
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).
Research Articles on MANF/ARMET
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Product Notes
The MANF/ARMET manf (Catalog #AAA2401957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MANF/ARMET Antibody reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MANF/ARMET can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:50-1:100 IHC: 1:50-1:100 IHC-P: 1:100 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MANF/ARMET manf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MANF/ARMET, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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