Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCF2L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit anti-Human, Mouse MCF2L Polyclonal Antibody | anti-MCF2L antibody
MCF2L Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCF2L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCF2L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
NCBI and Uniprot Product Information
Molecular Weight: 1137 (1123)
NCBI Description
This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Uniprot Description
ARHGEF14: Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus. Isoform 5 activates CDC42. Belongs to the MCF2 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs; GEFs, Rac/Rho; Oncoprotein
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: cytosol; extracellular space; lamellipodium; plasma membrane
Molecular Function: phosphatidylinositol binding; Rho guanyl-nucleotide exchange factor activity
Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of GTPase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction
Research Articles on MCF2L
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Product Notes
The MCF2L mcf2l (Catalog #AAA9129097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCF2L Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCF2L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:4000. Researchers should empirically determine the suitability of the MCF2L mcf2l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCF2L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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