Rabbit anti-Human, Mouse MAN2A2 Polyclonal Antibody | anti-MAN2A2 antibody

MAN2A2, ID (MAN2A2, MANA2X, Alpha-mannosidase 2x, Alpha-mannosidase IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) (Biotin)

Cat. Num. AAA6318167

• Gene Names: MAN2A2; MANA2X
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAN2A2; Polyclonal Antibody; ID (MAN2A2; MANA2X; Alpha-mannosidase 2x; Alpha-mannosidase IIx; Mannosidase alpha class 2A member 2; Mannosyl-oligosaccharide 1; 3-1; 6-alpha-mannosidase) (Biotin); 6-alpha-mannosidase; anti-MAN2A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAN2A2 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MAN2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-796 amino acids from the Central region of human MAN2A2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAN2A2 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).)

Immunohistochemistry (IHC)

(MAN2A2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAN2A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MAN2A2 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAN2A2 antibody

MAN2A2 catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Product Categories/Family for anti-MAN2A2 antibody

Antibodies; Enzymes; Glycosidase

References

Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008). Stelzl, U., et al. Cell 122(6):957-968(2005). Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004): Misago, M., et al. Proc. Natl. Acad. Sci. U.S.A. 92(25):11766-11770(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 51477716
• NCBI GeneID: 4122
• NCBI Accession #: NP_006113.2
• NCBI GenBank Nucleotide #: NM_006122.2
• UniProt Accession #: P49641
• Molecular Weight: 91,059 Da
• NCBI Official Full Name: alpha-mannosidase 2x
• NCBI Official Synonym Full Names: mannosidase alpha class 2A member 2
• NCBI Official Symbol: MAN2A2
• NCBI Official Synonym Symbols: MANA2X
• NCBI Protein Information: alpha-mannosidase 2x
• UniProt Protein Name: Alpha-mannosidase 2x
• Protein Family: Alpha-mannosidase
• UniProt Gene Name: MAN2A2
• UniProt Synonym Gene Names: MANA2X; Man IIx

Uniprot Description

MAN2A2: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. Belongs to the glycosyl hydrolase 38 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.114; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: alpha-mannosidase activity; carbohydrate binding; hydrolase activity, hydrolyzing N-glycosyl compounds; mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity; zinc ion binding

Biological Process: cellular protein metabolic process; mannose metabolic process; N-glycan processing; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein deglycosylation