Western Blot (WB)
(MAN2A2 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).)
Rabbit anti-Human, Mouse MAN2A2 Polyclonal Antibody | anti-Man2a2 antibody
MAN2A2, ID (MAN2A2, MANA2X, Alpha-mannosidase 2x, Alpha-mannosidase IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
Gene Names
Man2a2; MX; Man IIx; AI480988; 1700052O22Rik; 4931438M07Rik
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
MAN2A2; Polyclonal Antibody; ID (MAN2A2; MANA2X; Alpha-mannosidase 2x; Alpha-mannosidase IIx; Mannosidase alpha class 2A member 2; Mannosyl-oligosaccharide 1; 3-1; 6-alpha-mannosidase); Anti -MAN2A2; anti-Man2a2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Man2a2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
MAN2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-796 amino acids from the Central region of human MAN2A2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MAN2A2 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).)
Western Blot (WB)
(MAN2A2 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).)
Immunohistochemistry (IHC)
(MAN2A2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAN2A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MAN2A2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAN2A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MAN2A2 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MAN2A2 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Man2a2 antibody
MAN2A2 catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Product Categories/Family for anti-Man2a2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
130,649 Da
NCBI Official Full Name
Man2a2 protein
NCBI Official Synonym Full Names
mannosidase 2, alpha 2
NCBI Official Symbol
Man2a2
NCBI Official Synonym Symbols
MX; Man IIx; AI480988; 1700052O22Rik; 4931438M07Rik
NCBI Protein Information
alpha-mannosidase 2x; alpha mannosidase IIx; alpha-mannosidase IIx; mannosidase alpha class 2A member 2; mannosidase, alpha, class 2A, member 2; mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
UniProt Protein Name
Alpha-mannosidase 2x
Protein Family
UniProt Gene Name
Man2a2
UniProt Synonym Gene Names
Mana2x; Man IIx
UniProt Entry Name
MA2A2_MOUSE
Uniprot Description
Function: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway
By similarity.
Catalytic activity: Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.
Cofactor: Binds 1 zinc ion per subunit
By similarity.
Pathway: Protein modification; protein glycosylation.
Subunit structure: Homodimer; disulfide-linked
By similarity.
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
By similarity.
Sequence similarities: Belongs to the glycosyl hydrolase 38 family.
Research Articles on Man2a2
Similar Products
Product Notes
The Man2a2 man2a2 (Catalog #AAA649508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAN2A2, ID (MAN2A2, MANA2X, Alpha-mannosidase 2x, Alpha-mannosidase IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAN2A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Man2a2 man2a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAN2A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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