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Western Blot (WB) (MAGED4B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MAGED4B antibody detected the MAGED4B protein (arrow).)

Rabbit anti-Human MAGED4B Polyclonal Antibody | anti-MAGED4B antibody

MAGED4B, ID (MAGED4, KIAA1859, MAGED4A, MAGEE1, Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
MAGED4B; Polyclonal Antibody; ID (MAGED4; KIAA1859; MAGED4A; MAGEE1; Melanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen); Anti -MAGED4B; anti-MAGED4B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-MAGED4B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
MAGED4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-357 amino acids from the Central region of human MAGED4B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAGED4B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MAGED4B antibody detected the MAGED4B protein (arrow).)

Western Blot (WB) (MAGED4B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MAGED4B antibody detected the MAGED4B protein (arrow).)

Immunohistochemistry (IHC)

(MAGED4B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGED4B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (MAGED4B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGED4B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MAGED4B antibody
This gene is a member of the MAGED gene family. It is expressed only in brain and ovary among normal tissues, and two transcript variants of this gene are specifically expressed in glioma cells among cancer cells. This gene and the other MAGED genes are clustered on chromosome Xp11. Multiple alternatively spliced transcript variants have been found for this gene, however, the full-length nature of some variants has not been defined.
Product Categories/Family for anti-MAGED4B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,378 Da
NCBI Official Full Name
melanoma-associated antigen D4 isoform 3
NCBI Official Synonym Full Names
melanoma antigen family D, 4B
NCBI Official Symbol
MAGED4B
NCBI Protein Information
melanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen
UniProt Protein Name
Melanoma-associated antigen D4
UniProt Gene Name
MAGED4
UniProt Synonym Gene Names
KIAA1859; MAGED4A; MAGEE1
UniProt Entry Name
MAGD4_HUMAN

NCBI Description

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary, and some transcript variants of this gene are specifically expressed in glioma cells. This gene is clustered with other MAGED genes on chromosome Xp11. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

MAGE-D4B: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xp11

Research Articles on MAGED4B

Similar Products

Product Notes

The MAGED4B maged4 (Catalog #AAA6002790) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGED4B, ID (MAGED4, KIAA1859, MAGED4A, MAGEE1, Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGED4B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the MAGED4B maged4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGED4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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