Rabbit anti-Human RALBP1 Polyclonal Antibody | anti-RALBP1 antibody

RALBP1, NT (RALBP1, RLIP1, RLIP76, RalA-binding protein 1, 76kD Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1) (PE)

Cat. Num. AAA6340140

• Gene Names: RALBP1; RIP1; RLIP1; RLIP76
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RALBP1; Polyclonal Antibody; NT (RALBP1; RLIP1; RLIP76; RalA-binding protein 1; 76kD Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; Ral-interacting protein 1) (PE); Ral-interacting protein 1; anti-RALBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RALBP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RALBP1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 42-71 in the N-terminal region of human RALBP1, UniProt Accession #Q15311.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS649201.This demonstrates the RALBP1 antibody detected the RALBP1 protein (arrow).)

Related Product Information for anti-RALBP1 antibody

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).

Product Categories/Family for anti-RALBP1 antibody

Antibodies; Binding Proteins

References

Singhal, S.S., et al. Int. J. Cancer 126(6):1327-1338(2010). Singhal, S.S., et al. Cancer Lett. 283(2):152-158(2009). Awasthi, Y.C., et al. J Toxicol Environ Health B Crit Rev 12(7):540-551(2009). Singhal, S.S., et al. Cancer Res. 69(10):4244-4251(2009). Singhal, S.S., et al. Biochem. Pharmacol. 77(6):1074-1083(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5803145
• NCBI GeneID: 10928
• NCBI Accession #: NP_006779.1
• NCBI GenBank Nucleotide #: NM_006788.3
• UniProt Accession #: Q15311; D3DUI0
• Molecular Weight: 76063 MW
• NCBI Official Full Name: ralA-binding protein 1
• NCBI Official Synonym Full Names: ralA binding protein 1
• NCBI Official Symbol: RALBP1
• NCBI Official Synonym Symbols: RIP1; RLIP1; RLIP76
• NCBI Protein Information: ralA-binding protein 1
• UniProt Protein Name: RalA-binding protein 1
• Protein Family: RalA-binding protein
• UniProt Gene Name: RALBP1
• UniProt Synonym Gene Names: RLIP1; RLIP76; RalBP1; DNP-SG ATPase
• UniProt Entry Name: RBP1_HUMAN

NCBI Description

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Uniprot Description

RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 18p11.3

Cellular Component: membrane; cytosol

Molecular Function: protein binding; ATPase activity, coupled to movement of substances; ATPase activity; Rac GTPase binding; Ral GTPase binding; GTPase activator activity

Biological Process: regulation of GTPase activity; regulation of small GTPase mediated signal transduction; metabolic process; transport; small GTPase mediated signal transduction; chemotaxis; signal transduction