Rabbit anti-Human, Mouse MAFG Polyclonal Antibody | anti-MAFG antibody

MAFG, NT (MAFG, Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF) (APC)

Cat. Num. AAA6318001

• Gene Names: MAFG; hMAF
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAFG; Polyclonal Antibody; NT (MAFG; Transcription factor MafG; V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF) (APC); hMAF; anti-MAFG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAFG antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAFG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAFG.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MAFG Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAFG antibody detected the MAFG protein (arrow).)

Related Product Information for anti-MAFG antibody

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha-and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).

Product Categories/Family for anti-MAFG antibody

Antibodies; Transcription Factors

References

Wang, X., et al. PLoS ONE 5 (8), E11934 (2010): Berg, D.T., et al. J. Biol. Chem. 282(51):36837-36844(2007). Yamamoto, T., et al. Genes Cells 11(6):575-591(2006). Stelzl, U., et al. Cell 122(6):957-968(2005). Tramier, M., et al. Biophys. J. 83(6):3570-3577(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 49619228
• NCBI GeneID: 4097
• NCBI Accession #: NP_116100.2
• NCBI GenBank Nucleotide #: NM_032711.3
• UniProt Accession #: O15525
• Molecular Weight: 17,850 Da
• NCBI Official Full Name: transcription factor MafG
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G
• NCBI Official Symbol: MAFG
• NCBI Official Synonym Symbols: hMAF
• NCBI Protein Information: transcription factor MafG; basic leucine zipper transcription factor MafG
• UniProt Protein Name: Transcription factor MafG
• Protein Family: Transcription factor
• UniProt Gene Name: MAFG
• UniProt Entry Name: MAFG_HUMAN

NCBI Description

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]

Uniprot Description

MAFG: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm

Molecular Function: protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of cellular pH; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; adult behavior; regulation of epidermal cell differentiation; blood coagulation; regulation of cell proliferation