Rabbit anti-Human, Mouse MAFG Polyclonal Antibody | anti-MAFG antibody

MAFG, NT (MAFG, Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF) (Azide free) (HRP)

Cat. Num. AAA6318004

• Gene Names: MAFG; hMAF
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAFG; Polyclonal Antibody; NT (MAFG; Transcription factor MafG; V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF) (Azide free) (HRP); hMAF; anti-MAFG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MAFG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAFG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAFG.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAFG Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAFG antibody detected the MAFG protein (arrow).)

Related Product Information for anti-MAFG antibody

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha-and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).

Product Categories/Family for anti-MAFG antibody

Antibodies; Transcription Factors

References

Wang, X., et al. PLoS ONE 5 (8), E11934 (2010): Berg, D.T., et al. J. Biol. Chem. 282(51):36837-36844(2007). Yamamoto, T., et al. Genes Cells 11(6):575-591(2006). Stelzl, U., et al. Cell 122(6):957-968(2005). Tramier, M., et al. Biophys. J. 83(6):3570-3577(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 49619228
• NCBI GeneID: 4097
• NCBI Accession #: NP_116100.2
• NCBI GenBank Nucleotide #: NM_032711.3
• UniProt Accession #: O15525
• Molecular Weight: 17,850 Da
• NCBI Official Full Name: transcription factor MafG
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G
• NCBI Official Symbol: MAFG
• NCBI Official Synonym Symbols: hMAF
• NCBI Protein Information: transcription factor MafG; basic leucine zipper transcription factor MafG
• UniProt Protein Name: Transcription factor MafG
• Protein Family: Transcription factor
• UniProt Gene Name: MAFG
• UniProt Entry Name: MAFG_HUMAN

NCBI Description

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]

Uniprot Description

MAFG: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm

Molecular Function: protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of cellular pH; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; adult behavior; regulation of epidermal cell differentiation; blood coagulation; regulation of cell proliferation