Rabbit anti-Human CLCF1 Polyclonal Antibody | anti-CLCF1 antibody

CLCF1, NT (CLCF1, BSF3, CLC, NNT1, Cardiotrophin-like cytokine factor 1, B Cell-stimulating factor 3, Novel neurotrophin-1) (PE)

Cat. Num. AAA6286348

• Gene Names: CLCF1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLCF1; Polyclonal Antibody; NT (CLCF1; BSF3; CLC; NNT1; Cardiotrophin-like cytokine factor 1; B Cell-stimulating factor 3; Novel neurotrophin-1) (PE); B-cell-stimulating factor 3; Novel neurotrophin-1; anti-CLCF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CLCF1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 45-72 amino acids selected from the N-terminal region of human CLCF1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CLCF1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CLCF1 antibody detected the CLCF1 protein (arrow).)

Related Product Information for anti-CLCF1 antibody

This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-CLCF1 antibody

Antibodies; Neurotrophins

References

Rousseau, F., et al. J. Biol. Chem. 283(44):30341-30350(2008). Dagoneau, N., et al. Am. J. Hum. Genet. 80(5):966-970(2007). Rousseau, F., et al. Proc. Natl. Acad. Sci. U.S.A. 103(26):10068-10073(2006). Perret, D., et al. J. Biol. Chem. 279(42):43961-43970(2004). Burger, R., et al. Br. J. Haematol. 123(5):869-878(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 261245022
• NCBI GeneID: 23529
• NCBI Accession #: NP_001159684.1
• NCBI GenBank Nucleotide #: NM_001166212.1
• UniProt Accession #: Q9UBD9; Q6NZA4; B4DNT4
• Molecular Weight: 24,087 Da
• NCBI Official Full Name: cardiotrophin-like cytokine factor 1 isoform 2
• NCBI Official Synonym Full Names: cardiotrophin-like cytokine factor 1
• NCBI Official Symbol: CLCF1
• NCBI Official Synonym Symbols: CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
• NCBI Protein Information: cardiotrophin-like cytokine factor 1
• UniProt Protein Name: Cardiotrophin-like cytokine factor 1
• Protein Family: Cardiotrophin-like cytokine factor
• UniProt Gene Name: CLCF1
• UniProt Synonym Gene Names: BSF3; CLC; NNT1; BSF-3; NNT-1
• UniProt Entry Name: CLCF1_HUMAN

Uniprot Description

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding

Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of astrocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 2