IRAK4 active protein

IRAK4, Recombinant, Human (IRAK4, REN64, NY-REN-64, Interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, Interleukin-1 receptor associated kinase 4)

Cat. Num. AAA635777

• Gene Names: IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
• Purity: Highly Purified; 0.95
• Synonyms: IRAK4; Recombinant; Human (IRAK4; REN64; NY-REN-64; Interleukin-1 receptor-associated kinase 4; IRAK-4 mutated form 1; Interleukin-1 receptor associated kinase 4); IRAK4 active protein

• Purity/Purification: Highly Purified; 0.95
• Form/Format: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 400mM NaCl, 10% glycerol, 3mM DTT.

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.; Dilution: The enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher.
• Specific Activity: 18pmol/min/ug
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Testing Data

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Testing Data

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Related Product Information for IRAK4 active protein

(IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family. Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms. IRAK4 is the most recently discovered IRAK protein. IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways. Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges; this indicates that IRAK4 has an important role in innate immunity. Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB. IRAK-4 is believed to be required for the LPS-induced activation of antigenpresenting cells.

Product Categories/Family for IRAK4 active protein

Molecular Biology; MB-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 166795295
• NCBI GeneID: 51135
• NCBI Accession #: NP_001107654.1
• NCBI GenBank Nucleotide #: NM_001114182.2
• UniProt Accession #: Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589
• Molecular Weight: 77.5kD
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 4 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; IRAK-4; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4
• UniProt Entry Name: IRAK4_HUMAN

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; cytokine production; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency