Western Blot (WB)
(Staining (0.2ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat CBR1 Polyclonal Antibody | anti-CBR1 antibody
Goat anti-CBR1 Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2ug/ml.
Western Blot: Approx 30-35kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Human lung cancer cells (A549) with a previous batch.
Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2ug/ml.
Western Blot (WB)
(Staining (0.2ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.2ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]
Uniprot Description
CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.1.1.189; EC 1.1.1.184; EC 1.1.1.197
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: cytosol
Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity
Biological Process: cyclooxygenase pathway; drug metabolic process; epithelial cell differentiation; vitamin K metabolic process
Research Articles on CBR1
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Product Notes
The CBR1 cbr1 (Catalog #AAA421259) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CBR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CBR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2ug/ml. Western Blot: Approx 30-35kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Human lung cancer cells (A549) with a previous batch. Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2ug/ml. Researchers should empirically determine the suitability of the CBR1 cbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HGQFVSEKRV EQW. It is sometimes possible for the material contained within the vial of "CBR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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