Rabbit LYZ Polyclonal Antibody | anti-LYZ antibody

LYZ Polyclonal Antibody

Cat. Num. AAA3011257

• Gene Names: LYZ; LZM; LYZF1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LYZ; Polyclonal Antibody; LYZ Polyclonal Antibody; 1; 4 beta N acetylmuramidase C; 4-beta-N-acetylmuramidase C; EC 3.2.1.17; LYSC_HUMAN; Lysosyme; Lysozyme (renal amyloidosis); Lysozyme C; Lysozyme C precursor; LZM; Renal amyloidosis; anti-LYZ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LYZ polyclonal antibody detects endogenous levels of LYZ protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 148

• Applicable Applications for anti-LYZ antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:5000-1:10,000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:20-1:50
• Immunogen: Recombinant full length Human LYZ.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using LYZ antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse spleen using LYZ antibody.)

Related Product Information for anti-LYZ antibody

-The origins of the lysozyme proteins date back an estimated 400 to 600 million years. Generally, lysozyme genes are relatively small, roughly 10 kilobases in length, and composed of four exons and three introns. Originally a bacteriolytic defensive agent, the function of this family of proteins adapted to serve a digestive function in its present forms. Lysozymes in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Lysozyme C belongs to the glycosyl hydrolase 22 family, and newly identified relatives of Lysozyme C appear to possess anti-HIV activity, as well as preserved bacteriolytic function against Micrococcus lysodeikticus. Lysozyme C is capable of both hydrolysis and transglycosylation and also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. Lysozyme C defects are a cause of amyloidosis VIII, also called familial visceral or Ostertag-type amyloidosis.

NCBI and Uniprot Product Information

• NCBI GI #: 48428995
• NCBI GeneID: 4069
• UniProt Accession #: P61626
• Molecular Weight: ~ 17kDa
• NCBI Official Full Name: Lysozyme C
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM; LYZF1
• NCBI Protein Information: lysozyme C
• UniProt Protein Name: Lysozyme C
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM
• UniProt Entry Name: LYSC_HUMAN

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

Uniprot Description

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.

Protein type: EC 3.2.1.17; Endoplasmic reticulum; Secreted, signal peptide; Vesicle; Secreted; Hydrolase

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; lysozyme activity

Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response

Disease: Amyloidosis, Familial Visceral