Rabbit anti-Human Lysine, methylated Polyclonal Antibody
Lysine, methylated (Methyl-Lysine)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Lysine; methylated; Polyclonal Antibody; methylated (Methyl-Lysine); Anti -Lysine; anti-Lysine; methylated antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes proteins or peptides methylated on lysine residues (mono and di-methyllysine). Does not react with acetylated lysine. Species Crossreactivity: Multi-species.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 50% glycerol.
Applicable Applications for anti-Lysine, methylated antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA.
Dilution: Western Blot: 1:2000-1:5000. 0.2-0.5ug/ml was sufficient for detection of the methylated histone using melanoma cells in TBSt.
Dilution: Western Blot: 1:2000-1:5000. 0.2-0.5ug/ml was sufficient for detection of the methylated histone using melanoma cells in TBSt.
Immunogen
Methylated lysine (KLH)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Lysine, methylated antibody
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).
Product Categories/Family for anti-Lysine, methylated antibody
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Product Notes
The Lysine, methylated (Catalog #AAA628037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lysine, methylated (Methyl-Lysine) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lysine, methylated can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Dilution: Western Blot: 1:2000-1:5000. 0.2-0.5ug/ml was sufficient for detection of the methylated histone using melanoma cells in TBSt. Researchers should empirically determine the suitability of the Lysine, methylated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysine, methylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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