Testing Data
(Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK). Histones were isolated with cold 0.1N HCl extraction followed by method.Left lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt and blocked with synthetic methylated BSA 100 ug/ml).Right lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt.)
methylated Lysine (mono, di-mehtyl) Polyclonal Antibody
Anti-methylated Lysine (mono, di-mehtyl)
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity purified with epsilon N-methyl lysine on Agarose
Synonyms
methylated Lysine (mono; di-mehtyl); Polyclonal Antibody; Anti-methylated Lysine (mono; Anti-e-Methylated Lysine Antibody (Anti-MeK); anti-methylated Lysine (mono; di-mehtyl) antibody
Clonality
Polyclonal
Specificity
Recognize proteins or peptides methylated on lysine residues (mono, di-methylysine). Not cross-react with acetylated protein. Cross-reactivity with tri-methyllysine is not tested
Purity/Purification
Immunoaffinity purified with epsilon N-methyl lysine on Agarose
Form/Format
250 ug/ml in Tris-Phosphate buffer, 50% Glycerol.
Applicable Applications for anti-methylated Lysine (mono, di-mehtyl) antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Immunogen
Methylated KLH conjugates
Preparation and Storage
Store at -20°C, stable for up to one year.
Testing Data
(Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK). Histones were isolated with cold 0.1N HCl extraction followed by method.Left lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt and blocked with synthetic methylated BSA 100 ug/ml).Right lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt.)
Testing Data
(Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK). Histones were isolated with cold 0.1N HCl extraction followed by method.Left lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt and blocked with synthetic methylated BSA 100 ug/ml).Right lane: blot with 0.5 ug/ml of anti-Mek (MBS330027) in TBSt.)
Related Product Information for anti-methylated Lysine (mono, di-mehtyl) antibody
Affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a unique technique,. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on Agarose as affinity matrix. The antibodies could be utilized for detection, quantitation and isolation of proteins with methylated lysine residues.
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Product Notes
The methylated Lysine (mono, di-mehtyl) (Catalog #AAA330027) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's methylated Lysine (mono, di-mehtyl) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the methylated Lysine (mono, di-mehtyl) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "methylated Lysine (mono, di-mehtyl), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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