Western Blot (WB)
(Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilution Lane A: COCLO205 Whole Cell Lysate Lane B: RT-4 Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa)
Rabbit anti-Human LYPLAL1 Polyclonal Antibody | anti-LYPLAL1 antibody
Anti-LYPLAL1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
LYPLAL1; Polyclonal Antibody; Anti-LYPLAL1 Antibody; Rabbit Polyclonal; LYPLAL1 Antibody; Rabbit PAb; Antigen Affinity Purified; lysophospholipase-like 1; Anti-Q96AV0 Antibody; anti-LYPLAL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human LYPLAL1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-LYPLAL1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human LYPLAL1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human LYPLAL1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilution Lane A: COCLO205 Whole Cell Lysate Lane B: RT-4 Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa)
Western Blot (WB)
(Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilution Lane A: COCLO205 Whole Cell Lysate Lane B: RT-4 Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa)
Related Product Information for anti-LYPLAL1 antibody
Lysophospholipase-like 1 (LYPLAL1) is an uncharacterized metabolic serine hydrolase. Genome-wide association studies (GWAS) have detected an association between variants in or near the Lysophospholipase-like 1 (LYPLAL1) locus and metabolic traits, including central obesity, nonalcoholic fatty liver, and waist-to-hip ratio. GWAS identified several variants in the LYPLAL1 gene that are significantly associated with central obesity preferentially in females. LYPLAL1 is upregulated in the adipose tissue of obese patients and is shown to underlie a sex-specific locus for diet-induced obesity. The 1.7 A crystal structure of LYPLAL1 reveals a fold very similar to APT1, as expected, but features a shape of the active site that precludes binding of long-chain substrates. Biochemical data demonstrate that LYPLAL1 exhibits neither phospholipase nor triacylglycerol lipase activity, but rather accepts short-chain substrates.
References
Watson RA, et al. (2017) Lyplal1 is dispensable for normal fat deposition in mice. Dis Model Mech 10 (12): 1481-1488.Lei X, et al. (2015) Obesity associated lyplal1 gene is regulated in diet induced obesity but not required for adipocyte differentiation. Mol Cell Endocrinol 411 207-213.Burger M, et al. (2012) Crystal structure of the predicted phospholipase lyplal1 reveals unexpected functional plasticity despite close relationship to acyl protein thioesterases. J Lipid Res 53 (1): 43-50.Ahn K, et al. (2016) Discovery of a selective covalent inhibitor of lysophospholipase-like 1 (lyplal1) as a tool to evaluate the role of this serine hydrolase in metabolism. ACS Chem Biol 11 (9): 2529-2540.
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Product Notes
The LYPLAL1 (Catalog #AAA8108590) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LYPLAL1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LYPLAL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:50-1:200 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the LYPLAL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYPLAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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