Rabbit anti-Human Lymphocyte Activation Gene 3 Polyclonal Antibody | anti-LAG3 antibody

Lymphocyte Activation Gene 3 Antibody

Cat. Num. AAA9629249

• Gene Names: LAG3; CD223
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Lymphocyte Activation Gene 3; Polyclonal Antibody; Lymphocyte Activation Gene 3 Antibody; CD223; CD223 antigen; FDC protein; LAG-3; Lag3; LAG3_HUMAN; Lymphocyte activating 3; Lymphocyte activation gene 3; Lymphocyte activation gene 3 protein; Protein FDC; anti-LAG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Lymphocyte Activation Gene 3 Antibody detects endogenous levels of total Lymphocyte Activation Gene 3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LAG3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The KIEELE motif is required for interaction with downstream signaling molecules. Belongs to the LAG3 family.
• Post Translational Modifications: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner.
• Subunit Structure: Interacts with MHC class II (MHC-II); selectively recognizes stable complexes of peptide and MHC-II. Interacts with FGL1 (via the Fibrinogen C-terminal domain).
• Tissue Specificity: Primarily expressed in activated T-cells and a subset of natural killer (NK) cells.
• Function: Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear.May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.
• Note: Produced following cleavage of the main chain.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LAG3 antibody

Cell membrane; Single-pass type I membrane protein; Secreted; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 251757512
• NCBI GeneID: 3902
• UniProt Accession #: P18627; Q7Z643; A8K7T9
• Molecular Weight: 525
• NCBI Official Full Name: Lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN

NCBI Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; antigen binding; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation