Rabbit anti-Human Oncostatin M/OSM Polyclonal Antibody | anti-OSM antibody

Anti-Oncostatin M/OSM Antibody, Rabbit Polyclonal

Cat. Num. AAA8102859

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: Oncostatin M/OSM; Polyclonal Antibody; Anti-Oncostatin M/OSM Antibody; Rabbit Polyclonal; Oncostatin M/OSM Antibody; Rabbit PAb; oncostatin M; anti-OSM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Oncostatin M/OSM
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-OSM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Human Oncostatin M/OSM protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Oncostatin M/OSM (rh OSM). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-OSM rabbit polyclonal antibody at 1:500 dilutionLane A: Hu-T78 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-OSM antibody

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeI and typeII both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produces the fully active 196 residue form.

References

Tanaka M, et al. (2003) Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. Reviews of Physiology, Biochemistry and Pharmacology. 149: 39-52.Auguste P, et al. (1997) Signaling of type II oncostatin M receptor. J Biol Chem. 272 (25): 15760-4.Zarling JM, et al. (1986). Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci. 83 (24): 9739-43.

NCBI and Uniprot Product Information

• NCBI GI #: 10092621
• NCBI GeneID: 5008
• NCBI Accession #: NP_065391.1
• NCBI GenBank Nucleotide #: NM_020530.4
• UniProt Accession #: P13725; Q6FHP8; Q9UCP6
• Molecular Weight: 28,484 Da
• NCBI Official Full Name: oncostatin-M preproprotein
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: OSM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• Protein Family: Oncostatin
• UniProt Gene Name: OSM
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_HUMAN

NCBI Description

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; oncostatin-M receptor binding; cytokine activity

Biological Process: multicellular organismal development; negative regulation of hormone secretion; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; cell proliferation; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; response to heat; positive regulation of acute inflammatory response; regulation of growth; positive regulation of cell division; negative regulation of meiosis; positive regulation of cell proliferation; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; immune response