Rabbit anti-Human AAK1 Polyclonal Antibody | anti-AAK1 antibody

AAK1, CT (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048) (MaxLight 750)

Cat. Num. AAA6460802

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: AAK1; Polyclonal Antibody; CT (AP2-associated Protein Kinase 1; Adaptor-associated Kinase 1; KIAA1048) (MaxLight 750); anti-AAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AAK1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-AAK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human AAK1 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-AAK1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-AAK1 antibody

Antibodies; Protein Kinases

References

1. Henderson, D.M., Mol. Biol. Cell 18(7), 2698-2706 (2007). 2. Takahashi, T., Cancer Res. 66(24), 11932-11937 (2006). 3. Schmid, E.M., PLoS Biol. 4(9), E262 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 148277037
• NCBI GeneID: 22848
• NCBI Accession #: NP_055726.3
• NCBI GenBank Nucleotide #: NM_014911.3
• UniProt Accession #: Q2M2I8; Q4ZFZ3; Q53RX6; Q9UPV4
• Molecular Weight: 103,885 Da
• NCBI Official Full Name: AP2-associated protein kinase 1
• NCBI Official Synonym Full Names: AP2 associated kinase 1
• NCBI Official Symbol: AAK1
• NCBI Protein Information: AP2-associated protein kinase 1; adaptor-associated kinase 1
• UniProt Protein Name: AP2-associated protein kinase 1
• Protein Family: AP2-associated protein kinase
• UniProt Gene Name: AAK1
• UniProt Synonym Gene Names: KIAA1048
• UniProt Entry Name: AAK1_HUMAN

NCBI Description

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; NAK family

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: extrinsic to plasma membrane; clathrin-coated vesicle; leading edge; terminal button; coated pit

Molecular Function: protein serine/threonine kinase activity; protein binding; Notch binding; ATP binding

Biological Process: regulation of protein localization; protein stabilization; protein amino acid autophosphorylation; endocytosis; protein amino acid phosphorylation; positive regulation of Notch signaling pathway