Kappa-theraphotoxin-Hm1a Recombinant Protein | Kappa-TRTX-Hm1a recombinant protein

Recombinant Heteroscodra maculata Kappa-theraphotoxin-Hm1a

Cat. Num. AAA1172827

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Kappa-theraphotoxin-Hm1a; Recombinant Heteroscodra maculata Kappa-theraphotoxin-Hm1a; Kappa-TRTX-Hm1a recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-35aa; Full Length
• Sequence: ECRYLFGGCSSTSDCCKHLSCRSDWKYCAWDGTFS

• Species: Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

SDS-PAGE

Related Product Information for Kappa-TRTX-Hm1a recombinant protein

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 46577143
• UniProt Accession #: P60992
• Molecular Weight: 24 kDa
• NCBI Official Full Name: Kappa-theraphotoxin-Hm1a
• UniProt Protein Name: Kappa-theraphotoxin-Hm1a
• Protein Family: Kappa-theraphotoxin
• UniProt Gene Name: Kappa-TRTX-Hm1a
• UniProt Synonym Gene Names: Kappa-TRTX-Hm1a
• UniProt Entry Name: TX1_HETMC

Uniprot Description

Function: Inhibitor of voltage-gated potassium channels. It inhibits Kv2.1/KCNB1, Kv2.2/KCNB2, Kv4.1/KCND1, Kv4.2/KCND2 and Kv4.3/KCND3 potassium channels. May act by shifting activation of the channel to more depolarized voltages. The toxin binding sites may be situated on the S3-S4 extracellular linker of the channel. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. May need to partition into the membrane in order to bind to the channel. Intracerebroventricular injection into mice induces convulsions, spasms, tremors, and death. Ref.1

Subcellular location: Secreted.

Tissue specificity: Expressed by the venom gland.

Domain: The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin

By similarity.

Miscellaneous: IC50 is 280 nM on Kv4.1/KCND1, whereas percentage of current inhibition are 23% on Kv2.1/KCNB1 at 100 nM, 19.7% on Kv2.2/KCNB2 at 100 nM, 39% on Kv4.2/KCND2 at 300 nM, and 43% on Kv4.3/KCND3 at 300 nM (Ref.1).

Sequence similarities: Belongs to the huwentoxin-1 family.

Mass spectrometry: Molecular mass is 3994.59 Da from positions 1 - 35. Determined by MALDI. Ref.1