Rabbit anti-Human, Mouse LPXN Polyclonal Antibody | anti-LPXN antibody

LPXN, ID (LPXN, LDLP, Leupaxin) (FITC)

Cat. Num. AAA6316837

• Gene Names: LPXN; LDPL
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LPXN; Polyclonal Antibody; ID (LPXN; LDLP; Leupaxin) (FITC); Leupaxin; anti-LPXN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 386

• Applicable Applications for anti-LPXN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LPXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human LPXN.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LPXN Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the LPXN antibody detected the LPXN protein (arrow).)

Western Blot (WB)

(Western blot analysis of LPXN (arrow) using rabbit polyclonal LPXN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LPXN gene.)

Related Product Information for anti-LPXN antibody

The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-LPXN antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Tanaka, T., et al. Cancer Sci. 101(2):363-368(2010). Dai, H.P., et al. Genes Chromosomes Cancer 48(12):1027-1036(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Sundberg-Smith, L.J., et al. Circ. Res. 102(12):1502-1511(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758670
• NCBI GeneID: 9404
• NCBI Accession #: NP_004802.1
• NCBI GenBank Nucleotide #: NP_004802.1
• UniProt Accession #: O60711
• NCBI Official Full Name: leupaxin isoform 2
• NCBI Official Synonym Full Names: leupaxin
• NCBI Official Symbol: LPXN
• NCBI Official Synonym Symbols: LDPL
• NCBI Protein Information: leupaxin
• UniProt Protein Name: Leupaxin
• Protein Family: Leupaxin
• UniProt Gene Name: LPXN
• UniProt Synonym Gene Names: LDLP
• UniProt Entry Name: LPXN_HUMAN

NCBI Description

The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

leupaxin: a LIM domain-containing cytoplasmic protein preferentially expressed in hematopoietic cells. Homologous to the focal adhesion protein, paxillin. Associates with PYK2, a member of focal adhesion kinase family, it may participate in signaling at sites of adhesion. .

Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: cell projection; focal adhesion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; podosome; nucleus

Molecular Function: protein binding; zinc ion binding; transcription cofactor activity

Biological Process: regulation of cell adhesion mediated by integrin; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of B cell receptor signaling pathway; protein complex assembly; negative regulation of cell adhesion; signal transduction; cell adhesion