Western Blot (WB)
(Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (MBS1753677).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse kidney tissue lysatesLane 3: rat liver tissue lysatesLane 4: rat kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # MBS1753677) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD. )
Rabbit anti-Mouse, Rat LOC134147/CMBL Polyclonal Antibody | anti-Cmbl antibody
Anti-LOC134147/CMBL Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Western Blot (WB)
(Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (MBS1753677).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse kidney tissue lysatesLane 3: rat liver tissue lysatesLane 4: rat kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # MBS1753677) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (MBS1753677).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse kidney tissue lysatesLane 3: rat liver tissue lysatesLane 4: rat kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # MBS1753677) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of RAW264. 7 cells using anti-LOC134147/CMBL antibody (MBS1753677).Overlay histogram showing RAW264. 7 cells stained with MBS1753677 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LOC134147/CMBL Antibody (MBS1753677, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of RAW264. 7 cells using anti-LOC134147/CMBL antibody (MBS1753677).Overlay histogram showing RAW264. 7 cells stained with MBS1753677 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LOC134147/CMBL Antibody (MBS1753677, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
NCBI and Uniprot Product Information
Uniprot Description
CMBL: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. Belongs to the dienelactone hydrolase family.
Protein type: EC 3.1.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 15|15 B2
Cellular Component: cytoplasm
Molecular Function: hydrolase activity
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Product Notes
The Cmbl cmbl (Catalog #AAA1753677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LOC134147/CMBL Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LOC134147/CMBL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|. Researchers should empirically determine the suitability of the Cmbl cmbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOC134147/CMBL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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