Western Blot (WB)
(Figure 1. Western blot analysis of Cox6a2 using anti-Cox6a2 antibody (MBS1753843).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cox6a2 antigen affinity purified polyclonal antibody (Catalog # MBS1753843) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cox6a2 at approximately 11KD. The expected band size for Cox6a2 is at 11KD. )
Rabbit anti-Mouse, Rat Cox6a2 Polyclonal Antibody | anti-Cox6a2 antibody
Anti-Cox6a2 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse, Rat|
Western Blot (WB)
(Figure 1. Western blot analysis of Cox6a2 using anti-Cox6a2 antibody (MBS1753843).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cox6a2 antigen affinity purified polyclonal antibody (Catalog # MBS1753843) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cox6a2 at approximately 11KD. The expected band size for Cox6a2 is at 11KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Cox6a2 using anti-Cox6a2 antibody (MBS1753843).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cox6a2 antigen affinity purified polyclonal antibody (Catalog # MBS1753843) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cox6a2 at approximately 11KD. The expected band size for Cox6a2 is at 11KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of NRK cells using anti-Cox6a2 antibody (MBS1753843).Overlay histogram showing NRK cells stained with MBS1753843 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cox6a2 Antibody (MBS1753843, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of NRK cells using anti-Cox6a2 antibody (MBS1753843).Overlay histogram showing NRK cells stained with MBS1753843 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cox6a2 Antibody (MBS1753843, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Cox6a2 antibody (MBS1753843).Overlay histogram showing HEPA1-6 cells stained with MBS1753843 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cox6a2 Antibody (MBS1753843, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Cox6a2 antibody (MBS1753843).Overlay histogram showing HEPA1-6 cells stained with MBS1753843 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cox6a2 Antibody (MBS1753843, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Inoue, M., Uchino, S., Iida, A., Noguchi, S., Hayashi, S., Takahashi, T., Fujii, K., Komaki, H., Takeshita, E., Nonaka, I., Okada, Y., Yoshizawa, T., Van Lommel, L., Schuit, F., Goto, Y., Mimaki, M., Nishino, I. COX6A2 variants cause a muscle-specific cytochrome c oxidase deficiency. Ann. Neurol. 86: 193-202, 2019.
3. Quintens, R., Singh, S., Lemaire, K., De Bock, K., Granvik, M., Schraenen, A., Vroegrijk, I. O. C. M., Costa, V., Van Noten, P., Lambrechts, D., Lehnert, S., Van Lommel, L., and 10 others. Mice deficient in the respiratory chain gene Cox6a2 are protected against high-fat diet-induced obesity and insulin resistance. PLoS One 8: e56719, 2013. Note: Electronic Article.
NCBI and Uniprot Product Information
Uniprot Description
COX6A2: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 6A family.
Protein type: EC 1.9.3.1; Motility/polarity/chemotaxis; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; Mitochondrial
Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex IV
Molecular Function: cytochrome-c oxidase activity
Research Articles on Cox6a2
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Product Notes
The Cox6a2 cox6a2 (Catalog #AAA1753843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cox6a2 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cox6a2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse, Rat|. Researchers should empirically determine the suitability of the Cox6a2 cox6a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cox6a2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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