Rabbit anti-Human LNX1 Polyclonal Antibody | anti-LNX1 antibody

LNX1 Polyclonal Antibody

Cat. Num. AAA3011600

• Gene Names: LNX1; LNX; MPDZ; PDZRN2
• Reactivity: Human
• Applications: Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LNX1; Polyclonal Antibody; LNX1 Polyclonal Antibody; E3 ubiquitin-protein ligase LNX; Ligand of Numb-protein X 1; Numb-binding protein 1; PDZ domain-containing RING finger protein 2; LNX; PDZRN2; UNQ574/PRO1136; anti-LNX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LNX1 polyclonal antibody detects endogenous levels of LNX1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 632

• Applicable Applications for anti-LNX1 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:100
• Immunogen: Recombinant full length Human LNX1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using LNX1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-LNX1 antibody

LNX1 (Ligand of Numb Protein X1, LNX, PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 2, PDZRN2) is a RING finger and PDZ domain containing protein that associates with Numb, a phosphotyrosine-binding (PTB) domain-containing protein that functions as a cell fate determinant. Studies indicate that the protein contains an N-terminal PTB domain-binding motif and 4 PDZ domains. Northern blot analysis has detected the expression of LNX1 in heart, placenta, kindney, pancreas and brain tissues. By radiation hybrid mapping, the LNX gene was localized to human chromosome 4q12 between marker D4S1577 and marker D4S1594. Research suggests that the presence of multiple protein binding domains involved in signal transduction and association with Numb and SKIP may suggest an important role for LNX in tumorogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 187607071
• NCBI GeneID: 84708
• NCBI Accession #: NP_116011.2
• NCBI GenBank Nucleotide #: NP_116011.2
• UniProt Accession #: Q8TBB1
• Molecular Weight: ~ 81kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase LNX isoform b
• NCBI Official Synonym Full Names: ligand of numb-protein X 1
• NCBI Official Symbol: LNX1
• NCBI Official Synonym Symbols: LNX; MPDZ; PDZRN2
• NCBI Protein Information: E3 ubiquitin-protein ligase LNX
• UniProt Protein Name: E3 ubiquitin-protein ligase LNX
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: LNX1
• UniProt Synonym Gene Names: LNX; PDZRN2
• UniProt Entry Name: LNX1_HUMAN

NCBI Description

This gene encodes a membrane-bound protein that is involved in signal transduction and protein interactions. The encoded product is an E3 ubiquitin-protein ligase, which mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. This protein may play an important role in tumorogenesis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene, which is located on chromosome 17, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LNX1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; Adaptor/scaffold; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: cytoplasm

Molecular Function: ligase activity; PDZ domain binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: protein homooligomerization; protein ubiquitination during ubiquitin-dependent protein catabolic process