anti-Human LL37 / CAMP / Cathelicidin Polyclonal Antibody | anti-LL37 antibody

Rabbit Polyclonal to Human LL37 / CAMP / Cathelicidin

Cat. Num. AAA242692

• Gene Names: CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Peptide column affinity chromatography
• Synonyms: LL37 / CAMP / Cathelicidin; Polyclonal Antibody; Rabbit Polyclonal to Human LL37 / CAMP / Cathelicidin; CAMP | Antibacterial protein fall-39 | CAP-18 | CRAMP | FALL-39 | FALL39 | HCAP-18 | HSD26 | LL37 | CAP18; anti-LL37 antibody

• Host: LL37
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Peptide column affinity chromatography
• Form/Format: PBS containing 0.02% Sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LL37 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC - Paraffin (5 ug/ml); Western blot (1 ug/ml); (Optimal dilution to be determined by the researcher); ; Usage:; Cathelicidin antibody can be used for detection of Cathelicidin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 19 kDa Observed: 18 kDa
• Host Note: Cathelicidin antibody was produced in Rabbit
• Target: LL37 / Cathelicidin
• Immunogen Species: LL37 Cathelicidin antibody was raised against Human
• Antigen Type: Synthetic peptide
• Immunogen: LL37 / Cathelicidin antibody was raised against a 17 AA synthetic peptide near the center of the human Cathelicidin. The immunogen is located within aa 50-100 of Cathelicidin.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CAMP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Cathelicidin in Human Spleen cells with Cathelicidin antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of Cathelicidin in Human spleen tissue lysate with Cathelicidin antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 39753970
• NCBI GeneID: 820
• NCBI Accession #: NP_004336.2
• NCBI GenBank Nucleotide #: NM_004345.3
• UniProt Accession #: P49913; Q71SN9
• NCBI Official Full Name: cathelicidin antimicrobial peptide
• NCBI Official Synonym Full Names: cathelicidin antimicrobial peptide
• NCBI Official Symbol: CAMP
• NCBI Official Synonym Symbols: LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
• NCBI Protein Information: cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein
• UniProt Protein Name: Cathelicidin antimicrobial peptide
• UniProt Gene Name: CAMP
• UniProt Synonym Gene Names: CAP18; FALL39; CAP-18; hCAP-18
• UniProt Entry Name: CAMP_HUMAN

NCBI Description

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]

Uniprot Description

CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family.

Protein type: Cell cycle regulation; Vesicle; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol

Molecular Function: protein binding

Biological Process: interaction with host; defense response to Gram-positive bacterium; positive regulation of angiogenesis; innate immune response in mucosa; killing by host of symbiont cells; positive regulation of cell proliferation; defense response to bacterium; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium