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Western Blot (WB) (Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane).PGK2(arrow) was detected using the purified Pab.)

Rabbit PGK2 Polyclonal Antibody | anti-PGK2 antibody

PGK2 Antibody (C-term)

Gene Names
PGK2; PGKB; PGKPS; HEL-S-272; dJ417L20.2
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PGK2; Polyclonal Antibody; PGK2 Antibody (C-term); Phosphoglycerate kinase 2; Phosphoglycerate kinase; testis specific; PGKB; anti-PGK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-333 amino acids from the C-terminal region of human PGK2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
305-333
Sequence Length
417
Applicable Applications for anti-PGK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane).PGK2(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane).PGK2(arrow) was detected using the purified Pab.)
Related Product Information for anti-PGK2 antibody
PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
Product Categories/Family for anti-PGK2 antibody
References
Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999).
McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996).
Blanche, H., et al., Genomics 9(3):420-428 (1991).
McCarrey, J.R., et al., Nature 326(6112):501-505 (1987).
Michelson, A.M., et al., J. Biol. Chem. 260(11):6982-6992 (1985).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44796
NCBI Official Full Name
phosphoglycerate kinase 2
NCBI Official Synonym Full Names
phosphoglycerate kinase 2
NCBI Official Symbol
PGK2
NCBI Official Synonym Symbols
PGKB; PGKPS; HEL-S-272; dJ417L20.2
NCBI Protein Information
phosphoglycerate kinase 2
UniProt Protein Name
Phosphoglycerate kinase 2
Protein Family
UniProt Gene Name
PGK2
UniProt Synonym Gene Names
PGKB
UniProt Entry Name
PGK2_HUMAN

NCBI Description

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Uniprot Description

PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: phosphoglycerate kinase activity; ATP binding

Biological Process: sperm motility; glycolysis; phosphorylation

Research Articles on PGK2

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Product Notes

The PGK2 pgk2 (Catalog #AAA9204772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 305-333. The PGK2 Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's PGK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PGK2 pgk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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