Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat Lipin 3 Polyclonal Antibody | anti-LPIN3 antibody
Goat anti-Lipin 3 Antibody
Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3ug/ml.
Western Blot: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands).
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the lipin family of proteins, and all family members share strong homology in their C-terminal region. This protein is thought to form hetero-oligomers with other lipin family members, while one family member, lipin 1, can also form homo-oligomers. This protein contains conserved motifs for phosphatidate phosphatase 1 (PAP1) activity as well as a domain that interacts with a transcriptional co-activator. Lipin complexes act in the cytoplasm to catalyze the dephosphorylation of phosphatidic acid to produce diacylglycerol, which is the precursor of both triglycerides and phospholipids. Lipin complexes are also thought to regulate gene expression as transcriptional co-activators in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Uniprot Description
LPIN3: Regulates fatty acid metabolism. Magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis. Belongs to the lipin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.4
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: endoplasmic reticulum membrane; nucleus
Molecular Function: phosphatidate phosphatase activity; transcription coactivator activity
Biological Process: dephosphorylation; fatty acid catabolic process; phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; triacylglycerol biosynthetic process
Research Articles on LPIN3
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Product Notes
The LPIN3 lpin3 (Catalog #AAA420903) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Lipin 3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lipin 3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3ug/ml. Western Blot: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). Researchers should empirically determine the suitability of the LPIN3 lpin3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KPKQKEDAVA TD. It is sometimes possible for the material contained within the vial of "Lipin 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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