Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat AXIN1 Polyclonal Antibody | anti-AXIN1 antibody
Goat anti-AXIN1 Antibody
Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5ug/ml.
Western Blot: Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.
Protein type: Tumor suppressor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: beta-catenin destruction complex; cell cortex; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic microtubule; cytoplasmic vesicle; cytosol; lateral plasma membrane; nucleus; perinuclear region of cytoplasm; postsynaptic density
Molecular Function: beta-catenin binding; enzyme binding; GTPase activator activity; identical protein binding; protein binding; protein complex scaffold; protein homodimerization activity; protein kinase binding; receptor signaling complex scaffold activity; signal transducer activity; SMAD binding; ubiquitin protein ligase binding
Biological Process: activation of JNK activity; activation of protein kinase activity; apoptosis; axial mesoderm formation; cell death; cellular protein complex assembly; cytoplasmic microtubule organization and biogenesis; determination of left/right symmetry; dorsal/ventral axis specification; embryonic eye morphogenesis; forebrain anterior/posterior pattern formation; muscle cell development; negative regulation of fat cell differentiation; negative regulation of Wnt receptor signaling pathway; olfactory placode formation; optic placode formation; positive regulation of GTPase activity; positive regulation of JNK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein catabolic process; positive regulation of protein ubiquitination; positive regulation of transcription, DNA-dependent; positive regulation of ubiquitin-protein ligase activity; proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; Wnt receptor signaling pathway through beta-catenin
Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma
Research Articles on AXIN1
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Product Notes
The AXIN1 axin1 (Catalog #AAA420920) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AXIN1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's AXIN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5ug/ml. Western Blot: Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the AXIN1 axin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FEEKIIGKVE KVD. It is sometimes possible for the material contained within the vial of "AXIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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