Immunohistochemistry (IHC)
(Anti-LIG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Mouse anti-Human LIG1 / DNA Ligase 1 Monoclonal Antibody | anti-LIG1 antibody
Mouse Monoclonal [clone 10G12] (IgG2a,k) to Human LIG1 / DNA Ligase 1
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Immunohistochemistry (IHC)
(Anti-LIG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-LIG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Western blot of LIG1 expression in HeLa nuclear extract.)
Western Blot (WB)
(Western blot of LIG1 expression in HeLa nuclear extract.)
ELISA (EIA)
(Detection limit for recombinant GST tagged LIG1 is approximately 0.3 ng/ml as a capture antibody.)
ELISA (EIA)
(Detection limit for recombinant GST tagged LIG1 is approximately 0.3 ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Protein type: EC 6.5.1.1; DNA repair, damage; Ligase
Chromosomal Location of Human Ortholog: 19q13.2-q13.3
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; chromosome; nucleus
Molecular Function: DNA binding; DNA ligase activity; DNA ligase (ATP) activity; metal ion binding; ATP binding
Biological Process: anatomical structure morphogenesis; V(D)J recombination; mismatch repair; DNA strand elongation during DNA replication; DNA repair; lagging strand elongation; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; response to hydrogen peroxide; nucleotide-excision repair; cell division; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; DNA ligation during DNA repair; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; DNA metabolic process
Research Articles on LIG1
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Product Notes
The LIG1 lig1 (Catalog #AAA246399) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 10G12] (IgG2a,k) to Human LIG1 / DNA Ligase 1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIG1 / DNA Ligase 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000) Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. Researchers should empirically determine the suitability of the LIG1 lig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIG1 / DNA Ligase 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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