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Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with MBS648905. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).)
Rabbit anti-Mouse LGR5, loop2 Polyclonal Antibody
LGR5, loop2 (LGR5, GPR49, GPR67, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38)
Reactivity
Mouse
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Purified
Synonyms
LGR5; loop2; Polyclonal Antibody; loop2 (LGR5; GPR49; GPR67; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38); Anti -LGR5; anti-LGR5; loop2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LGR5 at ~100kD. Species Crossreactivity: mouse
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-LGR5, loop2 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC).
Immunofluorescence: 1:100, Flow Cytometry: 1:10-1:50, Western Blot: 1:1000
Immunofluorescence: 1:100, Flow Cytometry: 1:10-1:50, Western Blot: 1:1000
Application Notes
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, ELISA.
Immunogen
Synthetic peptide corresponding to aa689-719 from human LGR5, conjugated to KLH (O75473).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with MBS648905. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).)
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with MBS648905. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).)
Western Blot (WB)
(Western Blot analysis using MBS648905 in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
Western Blot (WB)
(Western Blot analysis using MBS648905 in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
Western Blot (WB)
(Western Blot analysis of MBS648905 in mouse cerebellum tissue lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis of MBS648905 in mouse cerebellum tissue lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using MBS648905 (bottom histogram) compared to a negative control cell (top histogram). FIRC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using MBS648905 (bottom histogram) compared to a negative control cell (top histogram). FIRC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LGR5, loop2 antibody
LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
Product Categories/Family for anti-LGR5, loop2 antibody
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Product Notes
The LGR5, loop2 (Catalog #AAA648905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGR5, loop2 (LGR5, GPR49, GPR67, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGR5, loop2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC). Immunofluorescence: 1:100, Flow Cytometry: 1:10-1:50, Western Blot: 1:1000. Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, ELISA. Researchers should empirically determine the suitability of the LGR5, loop2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR5, loop2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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