Rat Chemokine Like Factor Superfamily 4 ELISA Kit | CKLFSF4 elisa kit

Rat Chemokine Like Factor Superfamily 4 ELISA Kit

Cat. Num. AAA720818

• Gene Names: CMTM4; CKLFSF4
• Reactivity: Rat
• Synonyms: Chemokine Like Factor Superfamily 4; Rat Chemokine Like Factor Superfamily 4 ELISA Kit; CKLFSF4 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This CKLFSF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CKLFSF4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKLFSF4 elisa kit

Principle of the Assay: CKLFSF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CKLFSF4 antibody and an CKLFSF4-HRP conjugate. The assay sample and buffer are incubated together with CKLFSF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKLFSF4 concentration since CKLFSF4 from samples and CKLFSF4-HRP conjugate compete for the anti-CKLFSF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKLFSF4 from the sample, fewer sites are left to bind CKLFSF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKLFSF4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CKLFSF4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 30520333
• NCBI GeneID: 146223
• NCBI Accession #: NP_848933.1
• NCBI GenBank Nucleotide #: NM_178818.2
• Molecular Weight: 19,690 Da
• NCBI Official Full Name: CKLF-like MARVEL transmembrane domain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: CKLF-like MARVEL transmembrane domain containing 4
• NCBI Official Symbol: CMTM4
• NCBI Official Synonym Symbols: CKLFSF4
• NCBI Protein Information: CKLF-like MARVEL transmembrane domain-containing protein 4; chemokine-like factor super family 4; chemokine-like factor superfamily member 4
• UniProt Protein Name: CKLF-like MARVEL transmembrane domain-containing protein 4
• UniProt Gene Name: CMTM4
• UniProt Synonym Gene Names: CKLFSF4
• UniProt Entry Name: CKLF4_HUMAN

NCBI Description

This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies of signaling molecules. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CMTM4: a member of the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies of signaling molecules. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Chemokine

Chromosomal Location of Human Ortholog: 16q21-q22.1

Cellular Component: extracellular space; integral to membrane

Molecular Function: cytokine activity

Biological Process: chemotaxis