Rabbit anti-Human, Mouse LGR5 Polyclonal Antibody | anti-LGR5 antibody

LGR5 Antibody

Cat. Num. AAA856950

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: LGR5; Polyclonal Antibody; LGR5 Antibody; anti-LGR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of LGR5.
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid; 1*TBS (pH7.4), 0.5% BSA, 25% Glycerol, Preservative: 0.05% Sodium Azide.
• Sequence Length: 883

• Applicable Applications for anti-LGR5 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1,000-1:2,000; ICC: 1:50
• Cellular Localization: Cell Membrane Cell nucleus
• Positive Control: F9, NCCIT
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.

Western Blot

(Western blot analysis on F9 (A)and NCCIT (B) cell lysates using anti-LGR5 rabbit polyclonal antibody.)

Testing Data

(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)

Related Product Information for anti-LGR5 antibody

G-protein coupled receptor GPR49 (also known as Leucine-rich repeat-containing G-protein coupled receptor 5, Lgr5) is a stem cell marker of the intestinal epithelium and the hair follicule. It is expressed in the gonads, the adrenal gland, and in the brain, overexpressed in cancers of the ovary, colon and liver.

Product Categories/Family for anti-LGR5 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors."McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A.Cancer Biol. Ther. 5:419-426(2006). 2. "Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue."Becker L., Huang Q., Mashimo H.ScientificWorldJournal 8:1168-1176(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 469608414
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264155.1
• NCBI GenBank Nucleotide #: NM_001277226.1
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• Molecular Weight: 95kDa
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.