Rabbit anti-E Coli LexA, DNA-Binding Region Polyclonal Antibody | anti-lexA antibody

LexA, DNA-Binding Region (PE)

Cat. Num. AAA6485047

• Gene Names: lexA; ECK4035; exrA; JW4003; spr; tsl; umuA
• Reactivity: E Coli
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LexA; DNA-Binding Region; Polyclonal Antibody; DNA-Binding Region (PE); anti-lexA antibody

• Host: Rabbit
• Reactivity: E Coli
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the LexA portion of bait fusion proteins used in the yeast two-hybrid system. Species Crossreactivity: E. coli.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-lexA antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast lysate.; Applications are based on unconjugated antibody.; WB: 0.1-1ug/ml; Detects LexA-pRB in LexA-pRB transfected yeast lysate. LexA-pRB-transfected yeast cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
• Immunogen: His-tagged DNA binding domain of LexA protein corresponding to aa1-202.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot: LexA-pRB transfected yeast cell extract probed with MBS616282 at 0.1ug/ml.)

Product Categories/Family for anti-lexA antibody

Antibodies; Transcription Factors

References

1. Beranger, F., et al., Nucleic Acids Res. 25: 2035-2036 (1997). 2. Little, J.W., et al., Cell 29: 11-22 (1982).

NCBI and Uniprot Product Information

• NCBI GI #: 16131869
• NCBI GeneID: 948544
• NCBI Accession #: NP_418467.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A7C2; P03033; Q2M6R1
• Molecular Weight: 38.4 kDa
• NCBI Official Full Name: transcriptional repressor of SOS regulon
• NCBI Official Symbol: lexA
• NCBI Official Synonym Symbols: ECK4035; exrA; JW4003; spr; tsl; umuA
• NCBI Protein Information: transcriptional repressor of SOS regulon
• UniProt Protein Name: LexA repressor
• Protein Family: LexA repressor
• UniProt Gene Name: lexA
• UniProt Entry Name: LEXA_ECOLI

NCBI Description

LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533].

Uniprot Description

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).