Western Blot (WB)
(Western Blot analysis of LexA-pRB transfected yeast cell extract probed with MBS616282 (0.1ug/ml).)
Rabbit anti-E Coli LexA, DNA-Binding Region Polyclonal Antibody | anti-lexA antibody
LexA, DNA-Binding Region
Reactivity
E Coli
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
LexA; DNA-Binding Region; Polyclonal Antibody; Anti -LexA; anti-lexA antibody
Host
Rabbit
Reactivity
E Coli
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the LexA portion of bait fusion proteins used in the yeast two-hybrid system. Species Crossreactivity: E. coli.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-lexA antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested
Dilution: Western Blot: 0.1-1ug/ml; Detects LexA-pRB in LexA-pRB transfected yeast lysate. LexA-pRB-transfected yeast cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L2060 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast lysate.
Dilution: Western Blot: 0.1-1ug/ml; Detects LexA-pRB in LexA-pRB transfected yeast lysate. LexA-pRB-transfected yeast cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L2060 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast lysate.
Immunogen
His-tagged DNA binding domain of LexA protein corresponding to aa1-222.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of LexA-pRB transfected yeast cell extract probed with MBS616282 (0.1ug/ml).)
Western Blot (WB)
(Western Blot analysis of LexA-pRB transfected yeast cell extract probed with MBS616282 (0.1ug/ml).)
Western Blot (WB)
(Western Blot analysis of LexA-pRB -transfected yeast cell lysate using MBS616282 (1ug/ml).)
Western Blot (WB)
(Western Blot analysis of LexA-pRB -transfected yeast cell lysate using MBS616282 (1ug/ml).)
Related Product Information for anti-lexA antibody
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single-, double-, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
Product Categories/Family for anti-lexA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
LexA
NCBI Official Symbol
lexA
NCBI Protein Information
LexA repressor
Protein Family
Similar Products
Product Notes
The lexA (Catalog #AAA616282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LexA, DNA-Binding Region reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's LexA, DNA-Binding Region can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested Dilution: Western Blot: 0.1-1ug/ml; Detects LexA-pRB in LexA-pRB transfected yeast lysate. LexA-pRB-transfected yeast cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L2060 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast lysate. Researchers should empirically determine the suitability of the lexA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LexA, DNA-Binding Region, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
