Western Blot (WB)
(Western Blot: Sample: Rat Adrenal Gland Tissue.)
Rabbit anti-Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4) Polyclonal Antibody | anti-LILRB4 antibody
Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4)
Gene Names
Lilrb4a; HM18; ILT3; gp49; CD85K; Gp49b; LIR-5; Lilrb4
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Leukocyte Immunoglobulin Like Receptor Subfamily B; Member 4 (LILRB4); Polyclonal Antibody; Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B; anti-LILRB4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LILRB4. It has been selected for its ability to recognize LILRB4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.61mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GHLPKPI IWAEPGSVIA AYTSVITWCQ GSWEAQYYHL YKEKSVNPWD TQVPLETRNK AKFNIPSMTT SYAGIYKCYY ESAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFSCSSSIVF GRFILIQEGK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GHLPKPI IWAEPGSVIA AYTSVITWCQ GSWEAQYYHL YKEKSVNPWD TQVPLETRNK AKFNIPSMTT SYAGIYKCYY ESAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFSCSSSIVF GRFILIQEGK
Sequence Length
478
Applicable Applications for anti-LILRB4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LILRB4 (Gly24~Val206) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054754
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Adrenal Gland Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Adrenal Gland Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LILRB4, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LILRB4, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,049 Da
NCBI Official Full Name
leukocyte immunoglobulin-like receptor subfamily B member 4 isoform 1
NCBI Official Synonym Full Names
leukocyte immunoglobulin-like receptor, subfamily B, member 4A
NCBI Official Symbol
Lilrb4a
NCBI Official Synonym Symbols
HM18; ILT3; gp49; CD85K; Gp49b; LIR-5; Lilrb4
NCBI Protein Information
leukocyte immunoglobulin-like receptor subfamily B member 4
UniProt Protein Name
Leukocyte immunoglobulin-like receptor subfamily B member 4
Protein Family
UniProt Gene Name
Lilrb4
UniProt Synonym Gene Names
Gp49b
Uniprot Description
LILRB4: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5- signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 10 B3|10 25.5 cM
Cellular Component: extracellular exosome; integral component of membrane; membrane; plasma membrane
Biological Process: adaptive immune response; immune system process
Research Articles on LILRB4
Similar Products
Product Notes
The LILRB4 lilrb4 (Catalog #AAA2015392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LILRB4 lilrb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GHLPKPI IWAEPGSVIA AYTSVITWCQ GSWEAQYYHL YKEKSVNPWD TQVPLETRNK AKFNIPSMTT SYAGIYKCYY ESAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFSCSSSIVF GRFILIQEGK. It is sometimes possible for the material contained within the vial of "Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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