Leukocyte Ig-Like Receptor B4/LILRB4/CD85k/ILT3 Recombinant Protein | LILRB4/CD85k/ILT3 recombinant protein

Recombinant Mouse Leukocyte Ig-Like Receptor B4/LILRB4/CD85k/ILT3 (C-Fc)

Cat. Num. AAA2569322

• Gene Names: Lilrb4a; HM18; ILT3; gp49; CD85K; Gp49b; LIR-5; Lilrb4
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Leukocyte Ig-Like Receptor B4/LILRB4/CD85k/ILT3; Recombinant Mouse Leukocyte Ig-Like Receptor B4/LILRB4/CD85k/ILT3 (C-Fc); Leukocyte immunoglobulin-like receptor subfamily B member 4; Mast cell surface glycoprotein Gp49B; CD85k; Lilrb4; Gp49b; LILRB4/CD85k/ILT3 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 50 mM Tris-HCl, 100mM Glycine, pH7.5.
• Sequence: Gly24-Lys238

• Species: Mouse
• Tag: C-Fc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 is produced by our Mammalian expression system and the target gene encoding Gly24-Lys238 is expressed with a Fc tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for LILRB4/CD85k/ILT3 recombinant protein

Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC.

NCBI and Uniprot Product Information

• NCBI GI #: 7305097
• NCBI GeneID: 14728
• NCBI Accession #: NP_038560.1
• NCBI GenBank Nucleotide #: NM_013532.3
• UniProt Accession #: Q64281; Q64312
• Molecular Weight: 33,049 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 4 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B, member 4A
• NCBI Official Symbol: Lilrb4a
• NCBI Official Synonym Symbols: HM18; ILT3; gp49; CD85K; Gp49b; LIR-5; Lilrb4
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 4
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 4
• UniProt Gene Name: Lilrb4
• UniProt Synonym Gene Names: Gp49b
• UniProt Entry Name: LIRB4_MOUSE

Uniprot Description

LILRB4: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5- signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: integral to membrane; membrane; plasma membrane

Biological Process: adaptive immune response; immune system process