Rabbit anti-Human CD5 Monoclonal Antibody | anti-CD5 antibody

CD5

Cat. Num. AAA344192

• Reactivity: Human
• Synonyms: CD5; Monoclonal Antibody; anti-CD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

• Application Notes: 1:200
• Positive Control: Tonsil, Lymph Node
• Localization: Membranous
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Preparation and Storage: Store at 2-8 degree C.; To ensure stability, immediately replace vial back in the refrigerator after each use. When stored correctly, the antibody is stable until the expiry date indicated on the label.

Immunohistochemistry (IHC)

(CD5 on Tonsil)

Related Product Information for anti-CD5 antibody

Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a test panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.