Rabbit anti-Human TAF6L Polyclonal Antibody | anti-TAF6L antibody

TAF6L, NT (TAF6L, PAF65A, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65kD subunit 6L, PCAF-associated factor 65-alpha) (APC)

Cat. Num. AAA6353414

• Gene Names: TAF6L; PAF65A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF6L; Polyclonal Antibody; NT (TAF6L; PAF65A; TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65kD subunit 6L; PCAF-associated factor 65-alpha) (APC); PCAF-associated factor 65-alpha; anti-TAF6L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TAF6L antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF6L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human TAF6L.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAF6L Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the TAF6L antibody detected the TAF6L protein (arrow).)

Related Product Information for anti-TAF6L antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation.

Product Categories/Family for anti-TAF6L antibody

Antibodies; Enzymes; Polymerase

References

Clarke, D.L., et al. J. Immunol. 181(5):3503-3514(2008). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Lim, J., et al. Cell 125(4):801-814(2006). Pitkanen, J., et al. Biochem. Biophys. Res. Commun. 333(3):944-953(2005). Cavusoglu, N., et al. Proteomics 3(2):217-223(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 5453844
• NCBI GeneID: 10629
• NCBI Accession #: NP_006464.1
• NCBI GenBank Nucleotide #: NM_006473.3
• UniProt Accession #: Q9Y6J9; Q96HA6; B2RAT0
• Molecular Weight: 67,814 Da
• NCBI Official Full Name: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
• NCBI Official Synonym Full Names: TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
• NCBI Official Symbol: TAF6L
• NCBI Official Synonym Symbols: PAF65A
• NCBI Protein Information: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; PAF65-alpha; PCAF-associated factor 65 alpha; PCAF-associated factor 65-alpha; p300/CBP-associated factor (PCAF)-associated factor 65
• UniProt Protein Name: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
• Protein Family: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor
• UniProt Gene Name: TAF6L
• UniProt Synonym Gene Names: PAF65A; PAF65-alpha
• UniProt Entry Name: TAF6L_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF6L: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Belongs to the TAF6 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: histone deacetylase complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription factor activity; transcription initiation