Rabbit anti-Mouse Leptin Polyclonal Antibody | anti-Lep antibody

Leptin Antibody

Cat. Num. AAA840579

• Gene Names: Lep; ob; obese
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Leptin; Polyclonal Antibody; Leptin Antibody; LEP; OB; OBS; Obese Protein; anti-Lep antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-mouse Leptin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Sequence Length: 167

• Applicable Applications for anti-Lep antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-2 ug/ml). However, the optimal conditions should be determined individually. Other applications have not been tested.
• Immunogen: E. coli-expressed recombinant mouse Leptin.
• Preparation and Storage: Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Testing Data

Related Product Information for anti-Lep antibody

Background: Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acide residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 6678678
• NCBI GeneID: 16846
• NCBI Accession #: NP_032519
• NCBI GenBank Nucleotide #: NM_008493.3
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: Lep
• NCBI Official Synonym Symbols: ob; obese
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: Lep
• UniProt Synonym Gene Names: Ob
• UniProt Entry Name: LEP_MOUSE

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Hormone; Secreted; Secreted, signal peptide; Cell development/differentiation

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: protein binding; growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; positive regulation of JAK-STAT cascade; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; glucose homeostasis; negative regulation of metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; regulation of metabolic process; placenta development; central nervous system neuron development; cholesterol metabolic process; positive regulation of developmental growth; regulation of protein amino acid phosphorylation; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; fatty acid catabolic process; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; positive regulation of protein import into nucleus; glycerol biosynthetic process; insulin secretion; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; lipid metabolic process; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis