Mouse Leptin ELISA Kit | LEP elisa kit

Mouse Leptin ELISA Kit

Cat. Num. AAA8248446

• Gene Names: Lep; ob; obese
• Reactivity: Mouse
• Synonyms: Leptin; Mouse Leptin ELISA Kit; OB; OBS; Obese protein; Obesity factor; LEP elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Leptin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Leptin proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 167

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LEP elisa kit

Principle of the Assay: The Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Mouse ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and mouse DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of mouse chromosome 7q. The standard product used in this kit is recombinant mouse Leptin.

NCBI and Uniprot Product Information

• NCBI GI #: 6678678
• NCBI GeneID: 16846
• NCBI Accession #: NP_032519.1
• NCBI GenBank Nucleotide #: NM_008493.3
• UniProt Accession #: P41160
• Molecular Weight: 18,709 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: Lep
• NCBI Official Synonym Symbols: ob; obese
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: Lep
• UniProt Synonym Gene Names: Ob
• UniProt Entry Name: LEP_MOUSE

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Cell development/differentiation; Hormone; Secreted; Secreted, signal peptide

Cellular Component: cytoplasm; extracellular space

Molecular Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding; receptor binding

Biological Process: adult feeding behavior; angiogenesis; bile acid metabolic process; central nervous system neuron development; cholesterol metabolic process; eating behavior; energy reserve metabolic process; fatty acid beta-oxidation; fatty acid catabolic process; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process; hormone metabolic process; insulin secretion; interleukin-12 production; interleukin-6 production; intestinal absorption; leptin-mediated signaling pathway; leukocyte tethering or rolling; lipid metabolic process; negative regulation of apoptosis; negative regulation of appetite; negative regulation of autophagy; negative regulation of glucose import; negative regulation of metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vasoconstriction; ovulation from ovarian follicle; phagocytosis; placenta development; positive regulation of cell proliferation; positive regulation of developmental growth; positive regulation of insulin receptor signaling pathway; positive regulation of ion transport; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of myeloid cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of TOR signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; prostaglandin secretion; regulation of angiogenesis; regulation of blood pressure; regulation of bone remodeling; regulation of cell cycle; regulation of cholesterol absorption; regulation of endothelial cell proliferation; regulation of fat cell differentiation; regulation of gluconeogenesis; regulation of insulin secretion; regulation of metabolic process; regulation of natural killer cell activation; regulation of natural killer cell mediated cytotoxicity; regulation of natural killer cell proliferation; regulation of nitric-oxide synthase activity; regulation of protein amino acid phosphorylation; regulation of steroid biosynthetic process; response to dietary excess; response to insulin stimulus; sexual reproduction; T cell differentiation; tyrosine phosphorylation of STAT protein