Rabbit LBP Polyclonal Antibody | anti-LBP antibody

Anti-LBP Antibody

Cat. Num. AAA178622

• Gene Names: LBP; BPIFD2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: LBP; Polyclonal Antibody; Anti-LBP Antibody; Bpifd2; LBP1; Ly88; P18428; Lipopolysaccharide-binding protein; anti-LBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 481

• Applicable Applications for anti-LBP antibody: Western Blot (WB)
• Application Notes: Western Blot: 0.1-0.5ug/ml
• Notes: Tested Species: In-house tested species with positive results.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E Coli-derived human LBP recombinant protein (Position: L177-E446). Human LBP shares 68.5% and 69.6% amino acid (aa) sequence identity with mouse and rat LBP, respectively.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of LBP expression in rat thymus extract (lane 1), mouse thymus extract (lane 2), HELA whole cell lysates (lane 3) and K562 whole cell lysates (lane 4). LBP at 65KD was detected using rabbit anti- LBP Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-LBP antibody

Rabbit IgG polyclonal antibody for Lipopolysaccharide-binding protein(LBP) detection.
Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).

References

1. "Entrez Gene: LBP lipopolysaccharide binding protein".
2. Gray PW, Corcorran AE, Eddy RL Jr, Byers MG, Shows TB (March 1993). "The genes for the lipopolysaccharide binding protein (LBP) and the bactericidal permeability increasing protein (BPI) are encoded in the same region of human chromosome 20". Genomics. 15 (1): 188-90.

NCBI and Uniprot Product Information

• NCBI GI #: 31652249
• NCBI GeneID: 3929
• NCBI Accession #: NP_004130.2
• NCBI GenBank Nucleotide #: NM_004139.4
• UniProt Accession #: P18428; O43438; Q92672; Q9H403; Q9UD66; B2R938
• Molecular Weight: 53,384 Da
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein
• NCBI Official Symbol: LBP
• NCBI Official Synonym Symbols: BPIFD2
• NCBI Protein Information: lipopolysaccharide-binding protein
• UniProt Protein Name: Lipopolysaccharide-binding protein
• Protein Family: Lipopolysaccharide-binding protein
• UniProt Gene Name: LBP
• UniProt Synonym Gene Names: LBP

NCBI Description

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

Uniprot Description

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: lipopolysaccharide binding; protein binding; receptor binding

Biological Process: acute-phase response; cellular defense response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of tumor necrosis factor production; opsonization; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor production; response to lipopolysaccharide; toll-like receptor 4 signaling pathway