Flow Cytometry (FC/FACS)
(LARS Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Mouse LARS Polyclonal Antibody | anti-LARS antibody
LARS, NT (LARS, KIAA1352, Leucine-tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase) (APC)
Gene Names
LARS; LRS; LEUS; LFIS; ILFS1; LARS1; LEURS; PIG44; RNTLS; HSPC192; hr025Cl
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
LARS; Polyclonal Antibody; NT (LARS; KIAA1352; Leucine-tRNA ligase; cytoplasmic; Leucyl-tRNA synthetase) (APC); Leucine--tRNA ligase; Leucyl-tRNA synthetase; anti-LARS antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-LARS antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-213 amino acids from the N-terminal region of human LARS.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Flow Cytometry (FC/FACS)
(LARS Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(LARS Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(LARS Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LARS antibody detected the LARS protein (arrow).)
Western Blot (WB)
(LARS Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LARS antibody detected the LARS protein (arrow).)
Related Product Information for anti-LARS antibody
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.
Product Categories/Family for anti-LARS antibody
References
Pang, Y.L., et al. Biochemistry 48(38):8958-8964(2009). Seiradake, E., et al. J. Mol. Biol. 390(2):196-207(2009). Shin, S.H., et al. Exp. Mol. Med. 40(2):229-236(2008). Maeso, E., et al. Neuromuscul. Disord. 17(5):415-418(2007). Lue, S.W., et al. Biochemistry 46(15):4466-4472(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,295 Da
NCBI Official Full Name
leucine--tRNA ligase, cytoplasmic
NCBI Official Synonym Full Names
leucyl-tRNA synthetase
NCBI Official Symbol
LARS
NCBI Official Synonym Symbols
LRS; LEUS; LFIS; ILFS1; LARS1; LEURS; PIG44; RNTLS; HSPC192; hr025Cl
NCBI Protein Information
leucine--tRNA ligase, cytoplasmic; cytoplasmic leucyl-tRNA synthetase; cytosolic leucyl-tRNA synthetase; leucine tRNA ligase 1, cytoplasmic; leucine translase; leucine-tRNA ligase; leucyl-tRNA synthetase, cytoplasmic; proliferation-inducing gene 44
UniProt Protein Name
Leucine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
LARS
UniProt Synonym Gene Names
KIAA1352; LeuRS
UniProt Entry Name
SYLC_HUMAN
Uniprot Description
LARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs. Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: Translation; EC 6.1.1.4; Ligase; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: methionine-tRNA ligase activity; leucine-tRNA ligase activity; protein binding; ATP binding
Biological Process: leucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; regulation of translational fidelity; methionyl-tRNA aminoacylation; gene expression
Disease: Infantile Liver Failure Syndrome 1
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Product Notes
The LARS lars (Catalog #AAA6315229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LARS, NT (LARS, KIAA1352, Leucine-tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LARS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LARS lars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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