Goat anti-Human DUSP1 Polyclonal Antibody | anti-DUSP1 antibody

DUSP1, CT (Dual Specificity Protein Phosphatase 1, CL100, Dual Specificity Protein Phosphatase hVH1, HVH1, Mitogen-activated Protein Kinase Phosphatase 1, MAP Kinase Phosphatase 1, MKP1, MKP-1, Protein-tyrosine Phosphatase CL100, PTPN10, VH1)

Cat. Num. AAA641390

• Gene Names: DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DUSP1; Polyclonal Antibody; CT (Dual Specificity Protein Phosphatase 1; CL100; Dual Specificity Protein Phosphatase hVH1; HVH1; Mitogen-activated Protein Kinase Phosphatase 1; MAP Kinase Phosphatase 1; MKP1; MKP-1; Protein-tyrosine Phosphatase CL100; PTPN10; VH1); Anti -DUSP1; anti-DUSP1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human DUSP1. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-DUSP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 0.3-1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml
• Immunogen: Synthetic peptide corresponding to SYLQSPITTSPSC from the C-terminal of human DUSP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with DUSP1 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Chemiluminescence

(Antibody (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-DUSP1 antibody

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Product Categories/Family for anti-DUSP1 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 4758204
• NCBI GeneID: 1843
• NCBI Accession #: NP_004408.1
• NCBI GenBank Nucleotide #: NM_004417.3
• UniProt Accession #: P28562; Q2V508; D3DQL9
• Molecular Weight: 39,298 Da
• NCBI Official Full Name: dual specificity protein phosphatase 1
• NCBI Official Synonym Full Names: dual specificity phosphatase 1
• NCBI Official Symbol: DUSP1
• NCBI Official Synonym Symbols: HVH1; MKP1; CL100; MKP-1; PTPN10
• NCBI Protein Information: dual specificity protein phosphatase 1; MAP kinase phosphatase 1; protein-tyrosine phosphatase CL100; dual specificity protein phosphatase hVH1; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 1
• UniProt Protein Name: Dual specificity protein phosphatase 1
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP1
• UniProt Synonym Gene Names: CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1
• UniProt Entry Name: DUS1_HUMAN

NCBI Description

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/threonine phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: negative regulation of MAP kinase activity; response to light stimulus; response to cAMP; response to retinoic acid; negative regulation of MAPKKK cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; inactivation of MAPK activity; negative regulation of apoptosis