Human cytochrome P450, family 2, subfamily C, polypeptide 9 ELISA Kit | CYP2C9 elisa kit

Human Cytochrome P450 2C9, CYP2C9 ELISA Kit

Cat. Num. AAA939321

• Gene Names: CYP2C9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• Reactivity: Human
• Synonyms: cytochrome P450; family 2; subfamily C; polypeptide 9; Human Cytochrome P450 2C9; CYP2C9 ELISA Kit; Human Cytochrome P450 2C9 (CYP2C9) ELISA kit; CPC9; CYP2C; CYP2C10; MGC149605; MGC88320; P450IIC9; cytochrome P-450 S-mephenytoin 4-hydroxylase; subfamily IIC (mephenytoin 4-hydroxylase); polypeptide 10; subfamil; CYP2C9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat CYP1B1. No significant cross-reactivity or interference between rat CYP1B1 and analogues was observed.
• Sequence Length: 490

• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: 0.156 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2C9 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP1B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP1B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP1B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP1B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13699818
• NCBI GeneID: 1559
• NCBI Accession #: NP_000762.2
• NCBI GenBank Nucleotide #: NM_000771.3
• UniProt Accession #: P11712; P11713; Q16756; Q16872; Q5VX92; Q6IRV8; Q8WW80
• Molecular Weight: 55,628 Da
• NCBI Official Full Name: cytochrome P450 2C9
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily C, polypeptide 9
• NCBI Official Symbol: CYP2C9
• NCBI Official Synonym Symbols: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• NCBI Protein Information: cytochrome P450 2C9; cytochrome P-450MP; cytochrome P450 PB-1; microsomal monooxygenase; xenobiotic monooxygenase; flavoprotein-linked monooxygenase; cytochrome P-450 S-mephenytoin 4-hydroxylase
• UniProt Protein Name: Cytochrome P450 2C9
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C9
• UniProt Synonym Gene Names: CYP2C10
• UniProt Entry Name: CP2C9_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2C9: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.14.1; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Secondary Metabolites Metabolism - limonene and pinene degradation; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: (S)-limonene 6-monooxygenase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; (S)-limonene 7-monooxygenase activity; oxidoreductase activity; drug binding; steroid hydroxylase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; amide metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; drug metabolic process; monocarboxylic acid metabolic process; urea metabolic process

Disease: Coumarin Resistance