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Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Liver Tissue; Lane3: Mouse Thymus Tissue; Lane4: Mouse Spleen Tissue.)

Rabbit anti-Mouse Large Multifunctional Peptidase 2 Polyclonal Antibody | anti-LMP2 antibody

Polyclonal Antibody to Large Multifunctional Peptidase 2 (LMP2)

Gene Names
Psmb9; Lmp2; Lmp-2
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Large Multifunctional Peptidase 2; Polyclonal Antibody; Polyclonal Antibody to Large Multifunctional Peptidase 2 (LMP2); anti-LMP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LMP2. It has beenselected for its ability to recognize LMP2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TIMAVEFDG GVVVGSDSRV SAGTAVVNRV FDKLSPLHQR IFCALSGSAA DAQAIADMAA YQLELHGLEL EEPPLVLAAA NVVKNISYKY REDLLAHLIV AGWDQREGGQ VYGTMGGMLI RQPFTIGGSG SSYIYGYVDA AYKPGMTPEE CRRFTTNAIT LAMNRDGSSG GVIYLVTITA AGVDHRVILG DELPKFYDE
Sequence Length
219
Applicable Applications for anti-LMP2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Fragment
LMP2 (Thr22~Glu219)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant LMP2 (Thr22~Glu219) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041114
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Liver Tissue; Lane3: Mouse Thymus Tissue; Lane4: Mouse Spleen Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Liver Tissue; Lane3: Mouse Thymus Tissue; Lane4: Mouse Spleen Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant LMP2, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant LMP2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,397 Da
NCBI Official Full Name
proteasome subunit beta type-9
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2)
NCBI Official Symbol
Psmb9
NCBI Official Synonym Symbols
Lmp2; Lmp-2
NCBI Protein Information
proteasome subunit beta type-9
UniProt Protein Name
Proteasome subunit beta type-9
Protein Family
UniProt Gene Name
Psmb9
UniProt Synonym Gene Names
Lmp2; Ring12

Uniprot Description

PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Nuclear receptor co-regulator; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17 B1|17 17.98 cM

Cellular Component: cytoplasm; cytosol; nucleus; proteasome complex; proteasome core complex

Molecular Function: endopeptidase activity; hydrolase activity; peptidase activity; threonine endopeptidase activity

Biological Process: antigen processing and presentation; immune system process; proteolysis; proteolysis involved in cellular protein catabolic process

Research Articles on LMP2

Similar Products

Product Notes

The LMP2 psmb9 (Catalog #AAA2027723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Large Multifunctional Peptidase 2 (LMP2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Large Multifunctional Peptidase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LMP2 psmb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TIMAVEFDG GVVVGSDSRV SAGTAVVNRV FDKLSPLHQR IFCALSGSA A DAQAIADMAA YQLELHGLEL EEPPLVLAAA NVVKNISYKY REDLLAHLIV AGWDQREGG Q VYGTMGGMLI RQPFTIGGSG SSYIYGYVDA AYKPGMTPEE CRRFTTNAIT LAMNRDGSSG GVIYLVTITA AGVDHRVILG DELPKFYDE. It is sometimes possible for the material contained within the vial of "Large Multifunctional Peptidase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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