Human Large Multifunctional Peptidase 2 (LMP2) RTU ELISA Kit | LMP2 rtu elisa kit

Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit

Cat. Num. AAA4500795

• Gene Names: PSMB9; LMP2; PSMB6i; RING12; beta1i
• Reactivity: Human
• Synonyms: Large Multifunctional Peptidase 2 (LMP2); Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit; PSMB9; Proteasome(Prosome; Macropain)Subunit Beta Type 9; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Really interesting new gene 12 protein; LMP2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 219

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMP2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506205
• NCBI GeneID: 5698
• NCBI Accession #: NP_002791.1
• NCBI GenBank Nucleotide #: NM_002800.4
• UniProt Accession #: P28065; Q16523; Q5JNW4; B0V0T1
• Molecular Weight: 22,328 Da
• NCBI Official Full Name: proteasome subunit beta type-9
• NCBI Official Synonym Full Names: proteasome subunit beta 9
• NCBI Official Symbol: PSMB9
• NCBI Official Synonym Symbols: LMP2; PSMB6i; RING12; beta1i
• NCBI Protein Information: proteasome subunit beta type-9
• UniProt Protein Name: Proteasome subunit beta type-9
• Protein Family: Latent membrane protein
• UniProt Gene Name: PSMB9
• UniProt Synonym Gene Names: LMP2; PSMB6i; RING12

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.