Rabbit anti-Human, Mouse LAMP-2 Polyclonal Antibody | anti-Lamp2 antibody

LAMP-2 Antibody

Cat. Num. AAA150455

• Gene Names: Lamp2; Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: LAMP-2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: LAMP-2; Polyclonal Antibody; LAMP-2 Antibody; LAMP-2 Antibody: Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lysosomeassociated membrane glycoprotein 2; CD107 antigen-like family member B; anti-Lamp2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.
• Purity/Purification: LAMP-2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; LAMP-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 415

• Applicable Applications for anti-Lamp2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL and immunohistochemistry starting at 5 ug/mL.; Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.; ; Optimal dilutions for each application to be determined by the researcher.
• Homology: Predicted species reactivity based on immunogen sequence: Chicken: (75%), Rat: (75%), Bovine: (75%)
• Immunogen: LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2.; ; The immunogen is located within the last 50 amino acids of LAMP-2.
• Conjugate: Unconjugated
• Preparation and Storage: LAMP-2 antibody can be stored at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LAMP-2 in mouse liver tissue with LAMP-2 Antibody at 5 μg/mL.)

Related Product Information for anti-Lamp2 antibody

LAMP-2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 31543108
• NCBI GeneID: 16784
• NCBI Accession #: NP_054701
• NCBI GenBank Nucleotide #: NM_010685.4
• UniProt Accession #: P17047; Q3TXG5; Q8BSG8; A2A430
• Molecular Weight: 45,655 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform 2
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: Lamp2
• NCBI Official Synonym Symbols: Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2; lysosomal membrane glycoprotein 2; CD107 antigen-like family member B; lysosomal membrane glycoprotein type B
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: Lamp2
• UniProt Synonym Gene Names: Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B
• UniProt Entry Name: LAMP2_MOUSE

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: phagocytic vesicle membrane; membrane; lysosome; late endosome membrane; lysosomal membrane; late endosome; integral to membrane; plasma membrane; platelet dense granule membrane; endosome

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: protein stabilization