Rabbit anti-Human, Mouse LAMP-2 Polyclonal Antibody | anti-LAMP-2 antibody

LAMP-2 Antibody (CT)

Cat. Num. AAA840616

• Gene Names: LAMP2; LAMPB; CD107b; LAMP-2; LGP110
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: LAMP-2; Polyclonal Antibody; LAMP-2 Antibody (CT); Lysosome associated membrane protein 2; anti-LAMP-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG1
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: 100 ug (1 mg/ml) in 1X PBS containing 0.02% sodium azide.; Appearance: Colorless liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 411

• Applicable Applications for anti-LAMP-2 antibody: Western Blot (WB), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: Western Blot: 1 - 2 mug/ml, Immunocytochemistry: 10 mug/ml, ELISA. However, the optimal conditions should be determined individually.
• Immunogen: LAMP-2 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human LAMP-2.
• Positive Control: Western Blot: HepG2 cell lysateImmunocytochemistry: HepG2 cells
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-LAMP-2 antibody

Background: Autophagy, the process of bulk degradation of cellular proteins thromugh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.

Product Categories/Family for anti-LAMP-2 antibody

Apoptosis & Related products; Autophagy; Autophagy Antibodies Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 169790833
• NCBI GeneID: 3920
• NCBI Accession #: NP_001116078.1
• NCBI GenBank Nucleotide #: NM_001122606.1
• UniProt Accession #: P13473; Q16641; Q6Q3G8; Q96J30; Q99534; Q9UD93; A8K4X5; D3DTF0
• Molecular Weight: 45,170 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform C
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: LAMP2
• NCBI Official Synonym Symbols: LAMPB; CD107b; LAMP-2; LGP110
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• UniProt Gene Name: LAMP2
• UniProt Synonym Gene Names: LAMP-2; Lysosome-associated membrane protein 2
• UniProt Entry Name: LAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: phagocytic vesicle membrane; membrane; lysosomal membrane; late endosome membrane; lysosome; late endosome; integral to membrane; plasma membrane; platelet dense granule membrane

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: platelet activation; platelet degranulation; protein stabilization; blood coagulation

Disease: Danon Disease