Western Blot (WB)
(Western Blot: Sample: Recombinant LAMb4 Human.)
Rabbit anti-Human Laminin Beta 4 Polyclonal Antibody | anti-LAMb4 antibody
Polyclonal Antibody to Laminin Beta 4 (LAMb4)
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Laminin Beta 4; Polyclonal Antibody; Polyclonal Antibody to Laminin Beta 4 (LAMb4); anti-LAMb4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LAMb4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant LAMb4 (Asn1543~Cys1759) expressed in E.coli
Cross Reactivity
Human
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant LAMb4 Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LAMb4 Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
laminin subunit beta-4 isoform 1
NCBI Official Synonym Full Names
laminin subunit beta 4
NCBI Official Symbol
LAMB4
NCBI Protein Information
laminin subunit beta-4
UniProt Protein Name
Laminin subunit beta-4
Protein Family
UniProt Gene Name
LAMB4
Uniprot Description
LAMB4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q31.1
Cellular Component: basement membrane
Biological Process: cell adhesion
Research Articles on LAMb4
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Product Notes
The LAMb4 lamb4 (Catalog #AAA2027799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Laminin Beta 4 (LAMb4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin Beta 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LAMb4 lamb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin Beta 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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