Rabbit anti-Mouse p27Kip1 Polyclonal Antibody | anti-p27Kip1 antibody

p27Kip1, Phosphorylated (T197) (Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1) (APC)

Cat. Num. AAA6330165

• Gene Names: Cdkn1b; p27; Kip1; p27Kip1; AA408329; AI843786
• Reactivity: Mouse
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: p27Kip1; Polyclonal Antibody; Phosphorylated (T197) (Cdkn1b; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1) (APC); Cdkn1b; anti-p27Kip1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-p27Kip1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T197 of mouse p27Kip1.
• Conjugate: APC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Mouse p27Kip1 Antibody (Phospho T197) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-p27Kip1 antibody

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq].

Product Categories/Family for anti-p27Kip1 antibody

Antibodies; Protein Kinase Inhibitors

References

Singh, A., et al. Mol. Cell. Biol. 30(21):5145-5159(2010). Antico-Arciuch, V.G., et al. Oncogene 29(42):5678-5686(2010). Mitsuhashi, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16331-16335(2010). Zhang, Y., et al. Genes Dev. 24(16):1746-1757(2010). Wang, Y.Y., et al. J Mol Cell Biol 2(4):209-216(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 31542372
• NCBI GeneID: 12576
• NCBI Accession #: NP_034005.2
• NCBI GenBank Nucleotide #: NM_009875.4
• UniProt Accession #: P46414; Q8BG74
• Molecular Weight: 22,193 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1B
• NCBI Official Symbol: Cdkn1b
• NCBI Official Synonym Symbols: p27; Kip1; p27Kip1; AA408329; AI843786
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor p27
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• UniProt Gene Name: Cdkn1b
• UniProt Entry Name: CDN1B_MOUSE