Western Blot (WB)
(Western blot analysis of Lamin B1 expression in (1) Hela cell lysate; (2) Jurkat cell lysate; (3) Mouse brain lysate; (4) Rat heart lysate with Lamin B1 Antibody.)
Rabbit Lamin B1 Polyclonal Antibody | anti-LMNB antibody
Anti-Lamin B1 Rabbit pAb
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Lamin B1; Polyclonal Antibody; Anti-Lamin B1 Rabbit pAb; LMN; ADLD; LMN2; anti-LMNB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Lamin B1 Antibody detects endogenous levels of total Lamin B1
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
586
Applicable Applications for anti-LMNB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:3000-1:10000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
Immunogen
A synthesized peptide derived from human Lamin B1
Subcellular Location
Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of Lamin B1 expression in (1) Hela cell lysate; (2) Jurkat cell lysate; (3) Mouse brain lysate; (4) Rat heart lysate with Lamin B1 Antibody.)
Western Blot (WB)
(Western blot analysis of Lamin B1 expression in (1) Hela cell lysate; (2) Jurkat cell lysate; (3) Mouse brain lysate; (4) Rat heart lysate with Lamin B1 Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver, using Lamin B1 Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver, using Lamin B1 Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using Lamin B1 Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using Lamin B1 Antibody.)
Related Product Information for anti-LMNB antibody
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
lamin-B1 isoform 1
NCBI Official Synonym Full Names
lamin B1
NCBI Official Symbol
LMNB1
NCBI Official Synonym Symbols
LMN; ADLD; LMN2; LMNB
NCBI Protein Information
lamin-B1
UniProt Protein Name
Lamin-B1
UniProt Gene Name
LMNB1
UniProt Synonym Gene Names
LMN2; LMNB
NCBI Description
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Uniprot Description
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Research Articles on LMNB
Similar Products
Product Notes
The LMNB lmnb1 (Catalog #AAA477716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lamin B1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:3000-1:10000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50. Researchers should empirically determine the suitability of the LMNB lmnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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